Carbon use efficiency (CUE) is a key element in the vegetation carbon cycle, and determines how vegetation allocates carbon. Here, our research provides the spatio-temporal variations of CUE on the Tibetan Plateau (TP) based on ensemble simulations from 12 terrestrial ecosystem models. Moreover, the experimental design of simulations adds one time-varying driver at a time, thus quantitative analysis of the response of CUE to climate factors (i.e., temperature, precipitation and radiation), land use and land cover change (LULCC), and CO₂ fertilization can be investigated. Results show that average CUE value of the multi-model simulations (0.583 ± 0.064) on the TP is slightly lower than that derived from the satellite-based product, the Moderate Resolution Imaging Spectroradiometer (0.646). However, CUE varies greatly among models due to differences in simulating plant photosynthetic productivity and respiratory rate, with range of 0.489–0.661. LULCC and CO₂ fertilization contribute 4.24% and 0.79% of the annual mean CUE, respectively. Among the climatic factors, temperature and precipitation have positive correlations with CUE over most areas of the TP while solar radiation shows a negative impact.